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aggregation of vesicles

aggregation of vesicles

Interaction and aggregation of lipid vesicles (DLVO theory ...

1999-8-1 · Such vesicle aggregation studies have been done by several workers , , and the order of ability of ions to induce vesicle aggregation have been found for vesicles of different types of lipids , , . There exist various forces (electrostatic, van der Waals, hydration interactions, etc.) in stability of lipid vesicle suspension.

Self-limiting aggregation of phospholipid vesicles - Soft ...

Lipid vesicles are widely used as model systems to study biological membranes. The self-assembly of such vesicles into vesicle pairs provides further opportunity to study interactions between membranes. However, formation of vesicle pairs, while subsequently keeping their colloidal stability intact, is chall

Aggregation of phospholipid vesicles by water-soluble ...

1996-11-1 · Aggregation of large unilamellar vesicles required a lower polymer concentration, probably because aggregation occurs in a secondary minimum (without surface contact). In contrast to experiments in which the polymers were added directlyto the vesicles, dialysis ofthe vesicles against polymer-containing solutions did not induce aggregation.

Self-limiting aggregation of phospholipid vesicles - Soft ...

2019-8-20 · Vesicle aggregation experiments using cationic polymers were performed in a similar way to those with biotin and streptavidin: we started with single vesicles and stepwise added linker molecules to induce aggregation. The linker molecule here is the polycation poly-L-lysine (PLL). We continued the addition of PLL until unlimited aggregation was ...

Aggregation of phospholipid vesicles by water-soluble ...

The extent of aggregation was similar at 37 and 4 degrees C. Aggregation of large unilamellar vesicles required a lower polymer concentration, probably because aggregation occurs in a secondary minimum (without surface contact).

Frontiers | Calcium Dependent Reversible Aggregation of ...

Aggregation of E. coli vesicles was not observed when 2 mM MgCl 2 was introduced to the vesicle dispersion (White et al., 2000), as the authors used a phosphate buffer

Aggregation and fusion of phospholipid vesicles ...

The range of C11 values for Ca2+-induced aggregation and fusion of small unilamellar vesicles (SUV, 125 radius) composed of phosphatidylserine (PS) is 106 to 5 × 107 M-1 in the presence of Ca2+ concentrations from 1.15 to 2 mM, respectively. For larger PS vesicles (LUV, 500 radius) C11 = 6.5 × 107 M-1s-1 in the presence of 5 mM Ca2+.

Aggregation and fusion of phospholipid vesicles -

Membrane fusion and aggregation of phospholipid vesicles are reviewed and discussed. The fusion process is viewed as consisting of several stages: aggregation and close apposition of the particles, destabilization, and finally, merging of the bilayers. A procedure is presented which yields both the rate constant of the dimerization (C 11) and the rate constant for fusion of the ...

Effect of Surface Modification on Aggregation of ...

occur (10-12). In particular, small sonicated vesicles tend to aggregate and fuse below the phase transition temperature, re-sulting in an increase in vesicle size as a function of time (13-16). As these changes will ultimately affect the practical usefulness of phospholipid vesicles for

Trehalose prevents aggregation of exosomes and

2016-11-8 · However, extracellular vesicles suffer from aggregation and flocculation when stored in physiological saline solutions and degrade during freezing/thawing. It is currently advisable to proceed to ...

Aggregation of phospholipid vesicles by water-soluble ...

The extent of aggregation was similar at 37 and 4 degrees C. Aggregation of large unilamellar vesicles required a lower polymer concentration, probably because aggregation occurs in a secondary minimum (without surface contact).

Aggregation of phospholipid vesicles by water-soluble ...

1996-11-1 · Water-soluble polymers such as dextran and polyethylene glycol are known to induce aggregation and size growth of phospholipid vesicles. The present study addresses the dependence of these processes on vesicle size and concentration, polymer molecular weight, temperature, and compartmentalization of the vesicles and polymers, using static and dynamic light scattering.

Vesicle aggregation in aqueous mixtures of negatively ...

2019-12-9 · Vesicles aggregation occurs frequently in such systems, however, compared with liposome systems, investigations of vesicle aggregation and fusion in syn-thetic surfactant system were rather scarce [6,21,22]. Thus fur-ther systematic studies of environmental factors induced vesicle aggregation are necessary to understand how the factors work

Vesicle aggregation based on hydrophobic interactions ...

2015-8-27 · Vesicle aggregation has attracted much interest in the past few decades because of its applications in the modelling of biomembranes [1–3] and in drug and gene delivery [4, 5].It is known that the change of some experimental conditions can induce the aggregation of vesicles, for example, surfactant composition [6, 7], external additives [8–13], solution pH values [11, 14, 15], temperature ...

Phospholipid vesicle aggregation: effect of monovalent

2020-12-1 · Cation-induced aggregation of acidic phospholipid vesicles: the role of fatty acid unsaturation and cholesterol. Chemistry and Physics of Lipids 1991 , 57 (1) , 59-66.

Aggregation and fusion of phospholipid vesicles -

Membrane fusion and aggregation of phospholipid vesicles are reviewed and discussed. The fusion process is viewed as consisting of several stages: aggregation and close apposition of the particles, destabilization, and finally, merging of the bilayers. A procedure is presented which yields both the rate constant of the dimerization (C 11) and the rate constant for fusion of the ...

Aggregation of Cyanine Dye in Bilayer Vesicles of ...

2006-2-28 · result, J-aggregate in the bilayer vesicles are formed as Figure 3 indicates. J-aggregate of PIC-18 was also detected in more dilute mixtures of dye/DPPC. We have examined solutions of mixing ratios of 1:10, 2:10, and 3:10. In each of these solutions a significant amount of J-aggregate of the dye is formed during sonication.

Effect of Surface Modification on Aggregation of ...

occur (10-12). In particular, small sonicated vesicles tend to aggregate and fuse below the phase transition temperature, re-sulting in an increase in vesicle size as a function of time (13-16). As these changes will ultimately affect the practical usefulness of phospholipid vesicles for

Iron–Carbonyl Aqueous Vesicles (MCsomes) by Hydration

2015-11-13 · This aggregation subsequently enhances the hydration of the acyl CO groups and also induces the WCI of otherwise unhydrated terminal CO groups. The resultant metal–carbonyl aggregates have been proved to be bilayer vesicles with iron complexes exposed towards water and alkyl chains forming inner walls (MCsomes).

唐本忠 | 香港中文大学(深圳)

唐本忠教授于1982年和1988年分别在华南理工大学和日本京都大学取得学士学位和博士学位,于1989-1994年在加拿大多伦多大学进行了博士后研究并于Neos公司任高级研究员。1994年加入香港科技大学从事学术研究工作,2008年晋升为讲席教授。2021年,唐教授 ...

Electrostatic Control of Spontaneous Vesicle Aggregation ...

2019-5-25 · Aggregation of these vesicles was induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cross-linking receptor. Freeze−fracture transmission electron microscopy shows that almost no aggregation occurred in solutions with no added electrolyte, in which the Debye length was much ...

Vesicle aggregation in aqueous mixtures of negatively ...

2019-12-9 · Vesicles aggregation occurs frequently in such systems, however, compared with liposome systems, investigations of vesicle aggregation and fusion in syn-thetic surfactant system were rather scarce [6,21,22]. Thus fur-ther systematic studies of environmental factors induced vesicle aggregation are necessary to understand how the factors work

Aggregation and fusion of phospholipid vesicles -

Membrane fusion and aggregation of phospholipid vesicles are reviewed and discussed. The fusion process is viewed as consisting of several stages: aggregation and close apposition of the particles, destabilization, and finally, merging of the bilayers. A procedure is presented which yields both the rate constant of the dimerization (C 11) and the rate constant for fusion of the ...

Phospholipid vesicle aggregation: effect of monovalent

2020-12-1 · Cation-induced aggregation of acidic phospholipid vesicles: the role of fatty acid unsaturation and cholesterol. Chemistry and Physics of Lipids 1991 , 57 (1) , 59-66.

Effect of Surface Modification on Aggregation of ...

occur (10-12). In particular, small sonicated vesicles tend to aggregate and fuse below the phase transition temperature, re-sulting in an increase in vesicle size as a function of time (13-16). As these changes will ultimately affect the practical usefulness of phospholipid vesicles for

Aggregation of Cyanine Dye in Bilayer Vesicles of ...

2006-2-28 · result, J-aggregate in the bilayer vesicles are formed as Figure 3 indicates. J-aggregate of PIC-18 was also detected in more dilute mixtures of dye/DPPC. We have examined solutions of mixing ratios of 1:10, 2:10, and 3:10. In each of these solutions a significant amount of J-aggregate of the dye is formed during sonication.

Vesicle aggregation based on hydrophobic interactions ...

2015-8-27 · Vesicle aggregation has attracted much interest in the past few decades because of its applications in the modelling of biomembranes [1–3] and in drug and gene delivery [4, 5].It is known that the change of some experimental conditions can induce the aggregation of vesicles, for example, surfactant composition [6, 7], external additives [8–13], solution pH values [11, 14, 15], temperature ...

High-speed centrifugation induces aggregation of ...

2020-4-24 · SHORT COMMUNICATION High-speed centrifugation induces aggregation of extracellular vesicles Romain Linares1, Sisareuth Tan1,Ce´line Gounou1, Nicolas Arraud1 and Alain R. Brisson1,2* 1Molecular Imaging and NanoBioTechnology, University of Bordeaux, Pessac, France; 2Institut Universitaire de France, Paris, France

Interaction and aggregation of lipid vesicles (DLVO theory ...

1999-8-1 · Interaction and aggregation of acidic phospholipid (phosphatidylserine) vesicles were studied with variation of cation species and their concentrations in vesicle suspensions, and of vesicle sizes. Aggregation was determined by measuring turbidity of vesicle suspension. The experimental results of aggregation of vesicles induced by monovalent cations (Na+, K+, Cs+ and TMA+) were explained well ...

(PDF) Lipid vesicles trigger ?-synuclein aggregation by ...

a -Synuclein (a-syn) is a 140-residue intrinsically disordered protein that is involved in neuronal and synaptic vesicle plasticity, but its aggregation to form amyloid fibrils is the hallmark of ...